Optimal Feed Rate Control of Escherichia coli Fed- batch Fermentation

Optimal Feeding Trajectories Design for E. coli Fed-batch Fermentations

In this paper optimal control algorithms for two E. coli fed-batch fermentations are developed. Fed-batch fermentation processes of E. coli strain MC4110 and E. coli strain BL21(DE3)pPhyt109 are considered. Simple material balance models are used to describe the E. coli fermentation processes. The optimal feed rate control of a primary metabolite process is studied and a biomass production is u...

Identification of Yield Coefficients in an E. Coli Model - an Optimal Experimental Design Using Genetic Algorithms

An optimal experimental design for yield coefficients estimation in an unstructured growth model of fed-batch fermentation of E. coli is presented. The feed profile is designed by optimisation of a scalar function based on the Fischer Information Matrix. A genetic algorithm is proposed as the optimisation method due to its efficiency and independence on the initial values. Copyright 2004 IFAC K...

Mathematical Modelling and Variable Structure Control Systems for Fed-Batch Fermentation of Escherichia coli

In this paper some problems of the mathematical modelling and high control algorithms of feed-batch fermentation process of Escherichia coli are considered. An unstructured mathematical model for E. coli feed-batch fermentation is developed on the base of FIA measurements. Methodology for the models’ parameters estimation is proposed on the basis of nonlinear optimization methods. The model acc...

Fed-batch Cultivation of Recombinant Escherichia coli Producing Human Interferon-γ Under Controlled Specific Growth Rate

Implementation of a Specific Rate Controller in a Fed-batch E. Coli Fermentation

The specific growth rate is one of the most important process variables characterizing the state of microorganisms during fermentations mainly because the biosynthesis of many products of interest is often related with the values assumed by this parameter. In the particular case of the fed-batch operation of Escherichia coli for the production of recombinant proteins, it is important to maintai...